Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition Lac– strain, called lac3–, is linked to the Tn5 insertion. From a strain carrying the Tn5 insertion and lac3– mutation you isolate an F’ that caries a region of the chromosome that includes both Tn5 and the linked Lac region. Introduce this F’ into an F– strain carrying lac1– by selecting for Kanr. These merodiploids express ß-galactosidase normally. What does this result tell you about the relationship between the lac3– and lac1- mutations?  

#Question id: 10128

#Unit 6. System Physiology – Plant

PSII also involve in oxidation of water molecules, water is a very stable molecule that’s why  Oxidation of water to form molecular oxygen is very difficult, Many studies have provided a substantial amount of information about the process known;

which of the following are false ?

#Question id: 12759

#Unit 10. Ecological Principles

#Question id: 3831

#Unit 3. Fundamental Processes

State whether the following statements is true/false regarding DNA replication.

1. In E. coli replication begins at the origin of replication and proceeds in one direction until the entire circular DNA molecule has been copied.

2. The chromosome of the fruit fly (D. melanogaster) is about twice as large as the E. coli chromosome.

3. Prokaryotic DNA replication occurs in two steps. First, ATP provides a phosphate to the growing DNA chain. This is followed by addition of a nucleoside.

4. DNA polymerase III is the largest DNA polymerase in E. coli.

5. The β subunits of E. coli DNA polymerase form a sliding clamp that surrounds the DNA strands at the replication fork.

#Question id: 493

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following substrates cannot contribute to net gluconeogenesis in mammalian liver?

#Question id: 4084

#Unit 3. Fundamental Processes

The nuclear pore complex

a. is a large complex made of many different proteins.

b. is an octagonal, membrane-embedded structure with long filaments.

c. is required for transport of all molecules in or out of the nucleus.

d. binds to importin via FG-repeats.

e. hydrolyzes bound GTP to GDP