You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts^-1 to ts^-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.  

If the total size of the phage is 5 x 10⁴ bp and the total genetic map length of the phage is 100 m.u., about how far apart are the mutations in base pairs?

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#Question id: 21136

#Unit 13. Advances in Biotechnology

uptake of plasmid DNA into Streptomyces protoplasts is enhanced by__

TLS Online TPP Program

#Question id: 21137

#Unit 13. Advances in Biotechnology

Multiple cloning site for inserting target DNA on which placed to improve the productivity of antibiotics produced by Streptomyces spp___

TLS Online TPP Program

#Question id: 21138

#Unit 13. Advances in Biotechnology

Secondary metabolites production takes place in which phase of the bacterial growth curve?

TLS Online TPP Program

#Question id: 21139

#Unit 13. Advances in Biotechnology

Strain improvement for the large scale antibiotics production by using methods__

I) Gene transformation

II) Mutation and selection

III) Auxotophs mutants

TLS Online TPP Program

#Question id: 21140

#Unit 13. Advances in Biotechnology

In nutrition limitation condition, the bacterial growth curve reaches onto the Idiophase. In this phase which mechanism is responsible for enhancing the secondary metabolite production?

TLS Online TPP Program

#Question id: 21141

#Unit 13. Advances in Biotechnology

A vector contains the nitR gene under the control of the nitA promoter in a separate operon, the expression of vector is used, upon the addition of the inducer. The inducer–NitR protein complex activates the transcription of both the target gene(s) and nitR and increased level of NitR results in a very high level of production of the target protein(s). Which type of inducer using by nitR?