Various sterilization techniques used in plant tissue culture work;

Sterilization technique	Materials sterilized
A) Dry heat	i) Media, culture vessels, other glass plasticwarc, contaminated cultures
B) Autoclaving	ii) Heat labile compounds like GA3, ABA, zeatin, urea,·enzymes, etc.
C) Surface sterilization	iii) Empty glassware, instruments like scalpels, forceps, needles
D) Liquid (membrane filter of         0.45 μm or smaller pore size)	iv) All plant materials to be cultured

TLS Online TPP Program

#Question id: 21404

#Unit 12. Biochemical Engineering

Doubling the substrate concentration in the inlet of a CSTR (with cells following Monod growth kinetics) will, after reaching the new steady state, lead to:

TLS Online TPP Program

#Question id: 21405

#Unit 12. Biochemical Engineering

If 180 gm of glucose is consumed during cell growth and 132 gm of carbon dioxide is produced, then the fractional carbon flux towards biomass (assuming no product is formed and glucose is the sole carbon source) is:

TLS Online TPP Program

#Question id: 21406

#Unit 12. Biochemical Engineering

S. cerevisiae produces ethanol at a yield of 0.5 g/g glucose. The strain ferments 20g/l glucose in 24 hours. Calculate productivity of ethanol in this fermentation.

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#Question id: 21407

#Unit 12. Biochemical Engineering

E. coli was grown aerobically in batch fermentation. The initial concentration of cells was 1x 10³/ml and it reached 1x10⁶/ml in 10 hours. Calculate specific growth rate.

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#Question id: 21408

#Unit 12. Biochemical Engineering

In a fed batch process with a nongrowth product formation kinetics given by qp = β (a constant), in order to maximize product concentration and enhance metabolic flux towards product formation,

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#Question id: 21409

#Unit 12. Biochemical Engineering

Given that Power number is constant; then increasing the RPM of the impeller 3fold will increase the power consumption due to agitation by: