Transplantation of foreign chloroplasts into crop plants with inefficient photosynthetic system applied by

TLS Online TPP Program

#Question id: 13089

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 2’s plasmid is;

TLS Online TPP Program

#Question id: 23658

#Unit 13. Methods in Biology

How many recombinant clones must be screened in order to be 99% certain that a particular gene is found in a YAC library of human DNA where the average size of an insert is 500 kbp? The human genome size is about 3.2 x 10⁶ kbp. 

TLS Online TPP Program

#Question id: 92

#Unit 1. Molecules and their Interaction Relevant to Biology

Monosaccharide derivatives in which an amino group replaces one of the hydroxyl groups may have important roles in

TLS Online TPP Program

#Question id: 11337

#Unit 10. Ecological Principles

In which of the following biomes would we expect to find permafrost?

TLS Online TPP Program

#Question id: 10849

#Unit 10. Ecological Principles

Plasmodium is transmitted from human to human by the bite of a mosquito carrier. In this scenario plasmodium is the ________, the mosquito is the ________, and _______ is the role played by the human.