Practice Questions

TLS Online TPP Program

#Question id: 16129

5 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.
Describe the cis-acting elements in the GLN1 promoter that are evident from these experiments, giving both their position and as much of their function as you can deduce.

#Unit 13. Methods in Biology

Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain TATA, Deletion 6: sequence contain UAS Sequence -involved in activation.
Deletion1 : GLN3 function, Deletions 3, 4 : GLN2 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
Deletion1 : GLN2 function, Deletions 3, 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.
Deletion1, 3 and 4 : GLN3 function, contain UAS Sequence -involved in activation, Deletion 6: contain TATA sequence.