Practice Questions

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#Question id: 16134

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.

#Unit 13. Methods in Biology

PCR just amplifies RNA to which primers bind. One could imagine that the PCR primers could anneal to both sites and amplify both sites.
PCR just amplifies DNA to which primers bind. One could imagine that the PCR primers could anneal to one site and amplify only one site.
PCR just amplifies DNA to which primers bind, One could imagine that the PCR primers could anneal to both sites and amplify both sites.
PCR just amplifies protein to which primers bind. One could imagine that the PCR primers could anneal to both sites and amplify both sites.

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TLS Online TPP Program

#Question id: 12708

#Unit 8. Inheritance Biology

800 meiocytes with the genotype AaBb undergoes meiosis with 60% chance of crossing over with A and B. How many recombinant gamete form?

TLS Online TPP Program

#Question id: 12709

#Unit 8. Inheritance Biology

Which part is more recombinogenic and which part is more recombination frequency respectively?

TLS Online TPP Program

#Question id: 12710

#Unit 8. Inheritance Biology

When the two gene is present on the same chromosome and 50% chance of crossing over take place, then this is the case of ,

TLS Online TPP Program

#Question id: 12711

#Unit 6. System Physiology – Plant

Various sensors monitor ROS levels in cells; an increase in ROS activates a signal transduction network. ROS scavenging is carried out by antioxidant molecules ____X_________and by antioxidant enzymes such as____Z_________

TLS Online TPP Program

#Question id: 12712

#Unit 6. System Physiology – Plant

ROS using respiratory burst oxidase homolog (RBOH) or NADPH oxidase for Self- generation mechanism by some other processes , which are involved in ROS generation such as,

A) The amino-terminal cytoplasmic domain of RBOH proteins contains four serines (Ser) that can be phosphorylated by CDPKs and two EF hands that can bind calcium directly

B) As ROS generation such as H2O2, by putting electron for oxidising oxygen into the free radicals, these electron generation by oxidising NADPH and FADH2 in the cytosolic carboxy terminal of RBOH protein

C) The elevation of calcium and ROS levels during the early stages of the stress response activates protein kinases and phosphatases

D) ROS activated Ca2+ channel leads to Ca2+ influx from apoplastic space to cytosol

Arrange the correct order of following steps involve in ROS generation;

TLS Online TPP Program

#Question id: 12713

#Unit 6. System Physiology – Plant

Following are certain statements related to plants water deficit condition;

a) Inhibits Leaf Abscission

b) Enhances Root Extension into Deeper, Moist Soil

c) Limits Photosynthesis within the Chloroplast

d) decreases Resistance to Liquid-Phase Water Flow

e) Increases Wax Deposition on the Leaf Surface

f) Alters Energy Dissipation from Leaves

Which of the following combination given statements is correct