You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   

 

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

 

Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?

#Question id: 10496

#Unit 6. System Physiology – Plant

Blue light–stimulated stomatal opening is abolished by green light in the 500 to 600 nm region of the spectrum,what are these alphabet showing in the graph?

#Question id: 10497

#Unit 6. System Physiology – Plant

Blue light stimulated opening of stomata, closing of stomata is also takes place by many other such mutants and mechanisms such as,

a.) npq1 mutants leads to closing of stomata

b.) In high PPFD leads to conversion of violaxanthin to zeaxanthin which will  leads to closing of stomata

c.) Green light in the 500 to 600 nm region of the spectrum, abolishes the opening of stomata

d.) Mutant of phototropin 1 & 2 (PHOT1/PHOT2)  leads to closing of stomata

e.) Starch content in the guard cell decreases during closing of stomata at the end of the day

Which of the following given statements will be correct for closing of stomata?

#Question id: 10498

#Unit 6. System Physiology – Plant

Which one of the following inhibitor that inhibits blue light–stimulated stomatal opening and the inhibition is concentration dependent, while does not block red light–stimulated opening.

#Question id: 10499

#Unit 6. System Physiology – Plant

Stomata from intact, attached leaves of Arabidopsis illuminated with blue, red, and green light in a growth chamber, increases and decreases their aperture in response to light.

Find the CORRECT statements from the above graph.

i) Stomatal aperture increases when the green light is turned off, and close when the green light is turned on again

ii) Stomata from the phototropin-less double mutant phot1/phot2 respond to blue light and open further when green light is turned off

iii) stomata from the zeaxanthin less mutant npq1 do not respond to blue light and least effect to opening of the stomata further when green light is turned off

iv) the npq1 mutant and phot1/phot2 double mutant response indicate that the green reversal of the blue-light response requires zeaxanthin but also phototropin

#Question id: 10500

#Unit 6. System Physiology – Plant

Orange carotenoid protein (OCP) is involve in the photoprotection, these protein complex function as light intensity sensor provides;

#Question id: 29491

#Unit 6. System Physiology – Plant