Following statements are regarding to the some cancers harbor characteristic chromosome alterations. Chronic myelogenous leukemia (CML), a common leukemia in humans, was found to be associated with the Philadelphia chromosome.

A. Philadelphia chromosome is generated by a translocation between chromosomes 22 and 9. The two chromosomes exchange their terminal regions, which leads to a characteristic alteration in the size of chromosome 9.

B. At the breakpoint of this translocation, a new fusion protein, the BCR-ABL fusion, is generated.

C. The BCR-ABL fusion protein is a constitutively active kinase that phosphorylates multiple signal-transducing proteins.

D. If this translocation occurs in a hematopoietic cell in the bone marrow, the activity of the chimeric BCR-ABL oncogene dephosphorylate proteins thereby activating many intracellular signal-transducing proteins.

Which of the following statements are incorrect?

TLS Online TPP Program

#Question id: 12918

#Unit 13. Methods in Biology

Name the enzyme which helps in the DNA synthesis in the PCR.

TLS Online TPP Program

#Question id: 12919

#Unit 13. Methods in Biology

Which of the following statement is not correct about RT-PCR. 

TLS Online TPP Program

#Question id: 12920

#Unit 13. Methods in Biology

The development of isothermal systems such as the ____ DNA amplification system do away with the need for a thermal cycler and have the advantage of being able to be used outside the laboratory.

TLS Online TPP Program

#Question id: 12921

#Unit 13. Methods in Biology

Match the following:-

A. Gene probe production.              i. Novel proteins

B. Genome mapping.                       ii. PCR mutagenesis

C. Protein Engineering.                 iii. Sequence tagged sites

D. Gene Editing.                                iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Unit 13. Methods in Biology

 Match the following:-

1. Ligase chain reaction.              i. Mutation detection

2. Nucleic acid sequence based   amplification.                            ii.Non isothermal                   

3. Loop mediated isothermal amplification.                      iii. Viral detection

4. Branched DNA amplification.   iv.parasitic disease

TLS Online TPP Program

#Question id: 12923

#Unit 13. Methods in Biology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.