During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 

a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.

b) -1 frameshift is inserted after the amber so that no longer read as a stop.

c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.

d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.

Which of the following is the correct prediction about the frame shift mutation?

TLS Online TPP Program

#Question id: 15481

#Unit 12. Applied Biology

One of the approaches for the bacterial strain improvement for primary metabolite include

TLS Online TPP Program

#Question id: 15482

#Unit 12. Applied Biology

Over expression of primary metabolites are often achieved by

1. Frequency of RNA polymerase binding to the promoter region

2. Copy number increase of structural genes for these enzymes by genetic engineering

3. Chemically derived protein

Which combination below represent correct option?

TLS Online TPP Program

#Question id: 15483

#Unit 12. Applied Biology

A novel technique for strain improvement combining the advantage of multi-parental crossing allowed by DNA shuffling with the recombination of entire genomes.

TLS Online TPP Program

#Question id: 15484

#Unit 12. Applied Biology

Microbial process produces l-form of amino acids which makes the process advantageous as compared to synthetic processes. Strains employed for amino acid 1. 1. 1. production are 

1. wild-type strains 

2. auxotrophic mutants

3. regulatory mutants

4. auxotrophic regulatory mutants

Which of the following combination has correct options

TLS Online TPP Program

#Question id: 15485

#Unit 12. Applied Biology

Plasmid vector systems for cloning in which bacteria have been established, for amino acid production and related strains.

A. Corynebacterium

B. Micrococcus, 

C. Brevibacterium, 

D. Microbacterium

Choose the correct option.

TLS Online TPP Program

#Question id: 15486

#Unit 12. Applied Biology

Why glutamic acid accumulation in cell is not a good idea for Corynebacterium