TLS Online TPP Program
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TLS Online TPP Program
#Question id: 19842
#Unit 13. Methods in Biology
Primer-Dimer leads to the formation of ___
a) Inter-complemetary primers
b) Hybrid
c) Amplicons
TLS Online TPP Program
#Question id: 19843
#Unit 13. Methods in Biology
Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?
TLS Online TPP Program
#Question id: 19844
#Unit 13. Methods in Biology
Match the following column, which correctly represent the qPCR done by three methods and measure such as;
| COLUMN I | COLUMN II |
| A) SYBR green | i) Annealing |
| B) Taqman Assay | ii) Denaturation |
| C) Molecular beacon | iii) pH |
| iv) Amplification | |
| v) Extension |
TLS Online TPP Program
#Question id: 19845
#Unit 13. Methods in Biology
SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.
TLS Online TPP Program
#Question id: 19846
#Unit 13. Methods in Biology
Variants of PCR has been developed on the basis of___
TLS Online TPP Program
#Question id: 19865
#Unit 13. Methods in Biology
Match the following activity of variants in Column I with their variants of PCR in Column II given below;
| COLUMN I | COLUMN II |
| A) Enhance efficiency | i) Multiplex PCR |
| B) Sequence specific amplification | ii) Hot-start PCR |
| C) Mutational analysis | iii) Megaprimer PCR |
| iv) qPCR with SYBR | |
| v) AS-PCR | |
| vi) RLM-RACE |
