At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

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#Question id: 13083

#Unit 13. Methods in Biology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 

(1) both ends cleaved by PstI (Pst-Pst), 

(2) both ends cleaved by MseI (Mse-Mse), and 

(3) one end generated by each of the two enzymes (Pst-Mse). 

the most frequent fragments in decreasing order,

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#Question id: 12031

#Unit 7. System Physiology – Animal

Obesity in humans is most clearly linked to ________.

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#Question id: 32132

#Unit 1. Molecules and their Interaction Relevant to Biology

For guanine, the pKa value is 

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#Question id: 23123

#Unit 2. Cellular Organization

Which technique is used to analyzed homology between two gene

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#Question id: 11379

#Unit 10. Ecological Principles

Which of the following is NOT a prediction arising out of Wilson-MacArthur's -Theory of Island Biogeography?