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TLS Online TPP Program

#Question id: 18706

Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly

DNA polymeraseSource
a.Tmai.Thermotoga maritima
b.Deep vent tmii.Pyrococcus furiosus
c.Tliiii.Thermococcus litoralis
d.Pfuiv.Pyrococcus sp
e.Pwov.Pyrococcus woesi

#Unit 14. Methods in Biology
  1. a – iv, b – ii, c – iii, d – i, e - v
  2. a – ii, b – i, c – iii, d – iv, e - v
  3. a – i, b – v, c – iii, d – iv, e - ii
  4. a – i, b – ii, c – iii, d – iv, e - v
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TLS Online TPP Program

#Question id: 41076

#Unit 12. Biochemical Engineering

Q. A bacterial strain has μmax = 0.8 h^-1 and Ks = 0.2 g/L. What is the specific growth rate when the residual substrate concentration is 0.05 g/L?
TLS Online TPP Program

#Question id: 41077

#Unit 12. Biochemical Engineering

Q. In a chemostat operating at steady state, the specific growth rate of a bacterial culture is 0.2 h⁻¹. The true biomass yield coefficient on glucose (YX/S) is 0.5 g/g, and the cell maintenance coefficient (mS) is 0.05 g/(g·h).
The specific glucose uptake rate (qS) of the culture in g/(g·h) is ________ .
TLS Online TPP Program

#Question id: 41078

#Unit 12. Biochemical Engineering

A bacterial culture is growing in a batch reactor following Monod growth kinetics. The maximum specific growth rate (umax) of the culture is 0.8 h⁻¹, and the Monod saturation constant (KS) is 0.2 g/L.
If the current substrate concentration (S) in the reactor is 0.8 g/L, the specific growth rate (mu) of the culture in h⁻¹ is ________ (Round off to two decimal places).
TLS Online TPP Program

#Question id: 41079

#Unit 12. Biochemical Engineering

Q. A fed-batch bioreactor is operated under a quasi-steady state for the substrate, meaning the total amount of unconsumed substrate in the reactor remains constant over time (d(SV)/dt = 0).The nutrient feed is supplied at a constant flow rate (F) of 2.0 L/h, and the substrate concentration in the feed (Sf) is 10.0 g/L. At a specific time during the process, the total mass of the cells in the reactor (XV) is measured to be 40.0 g. Assuming there is no biomass in the incoming feed, calculate the specific substrate consumption rate (qS) of the culture in g/(g·h). _________ (Answer in integer or decimal).
TLS Online TPP Program

#Question id: 41080

#Unit 12. Biochemical Engineering

Q.A continuous stirred-tank bioreactor (chemostat) with a working volume (V) of 5.0 L is being used to cultivate a bacterial strain. The process operates at steady state. If the operator wants to maintain the specific growth rate (μ) of the bacteria at 0.25 h^-1, what should be the volumetric feed flow rate (F) of the nutrients in L/h? _________ (Answer in integer or decimal).
TLS Online TPP Program

#Question id: 41081

#Unit 12. Biochemical Engineering

  Q. In a stirred tank bioreactor, the specific interfacial surface area (a) is defined by the relationship a = 6ε/db. If an adjustment in the sparging system reduces the mean bubble diameter (db) by half while the gas holdup (ε) remains constant, how will this affect the specific interfacial surface area?A) It decreases by half.B) It remains unchanged.C) It doubles.D) It increases by a factor of four.how will this affect the specific interfacial surface area?