TLS Online TPP Program

#Question id: 18737


RNase S1 method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector?

#Unit 13. Methods in Biology
  1. Blunt ended ligation
  2. Addition of linkers
  3. Using appropriate restriction enzymes
  4. All the methods can be used equivalently
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TLS Online TPP Program

#Question id: 19868

#Unit 13. Methods in Biology

Which one of the following statements about FRET is correct?
a) FRET is possible only when the distance between donor and acceptor are within 1 to 10 A0
b) For FRET, GFP chromophore contain tripeptide sequence is -Gly-Phe-Pro-
c) qPCR based on FRET
d) Efficiency of FRET is directly proportional to the sixth power of the distance between donor and acceptor. 

TLS Online TPP Program

#Question id: 20981

#Unit 12. Applied Biology

For constructing the glucose sensor, which of the following is used as a gel?

TLS Online TPP Program

#Question id: 15262

#Unit 2. Cellular Organization

A media which makes easier to distinguish colonies of the desired organism from other colonies growing on the same plate.

TLS Online TPP Program

#Question id: 28430

#Unit 5. Developmental Biology

which of the following cells form a third layer that lies between the hypoblast and the epiblast cells ?

TLS Online TPP Program

#Question id: 16133

#Unit 13. Methods in Biology

You are running a human assisted reproduction clinic and providing state-of-the-art genetic diagnostic services. A married couple who already had a child with cystic fibrosis approach you because they wish to have another child, but only if they can be assured that the child will not have cystic fibrosis. You genotype the woman and discover that she is a heterozygote for Del508, the most common mutation causing cystic fibrosis. You suggest that the couple consider first polar body testing, in which several unfertilized oocytes (each with its first polar body) are retrieved from the woman, the first polar bodies are removed, and PCR tests are conducted on DNA from each of the first polar bodies. The couple agrees, and you obtain the following results:

 
Given the couple's desire to have a child without cystic fibrosis, which oocytes would you employ in vitro fertilization?