TLS Online TPP Program

#Question id: 18794


Which of the following compound is rapidly phosphorylated and incorporated in its place into newly synthesized DNA and is used to detect replicating cells.

#Unit 13. Methods in Biology
  1. The MTT Assay
  2. carbon method 
  3. EDTA
  4. bromodeoxyuridine (BrdU)
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TLS Online TPP Program

#Question id: 24490

#Unit 3. Fundamental Processes

There are three type of Splicing signals 1. Major U2-type or GU-AG introns 2. Minor U12-type or AU-AC introns & 3. Minor U12-type with GU-AG introns (hybrid type), which of the following is responsible for the recognition of branch point sequence UACUAAC?

TLS Online TPP Program

#Question id: 24491

#Unit 3. Fundamental Processes

There are three type of Splicing signals 1. Major U2-type or GU-AG introns 2. Minor U12-type or AU-AC introns & 3. Minor U12-type with GU-AG introns (hybrid type), which of the following is responsible for the recognition of branch point sequence CCUUAACU

TLS Online TPP Program

#Question id: 24492

#Unit 3. Fundamental Processes

Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site. So, in below table the complex and their events are given match them correctly .

TLS Online TPP Program

#Question id: 24493

#Unit 3. Fundamental Processes

Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site. 
1. U2 addition to base pair with the branch site in the presence of ATP
2. U1 and U4 release Formation of the catalytic center in which U6 base pairs with the 5' splice site; U6 also base pairs with U2; U2 remains base paired with the branch site; U5 interacts with both exons through its loop.

So, 1 and 2 are the event of complex formation which are 

TLS Online TPP Program

#Question id: 24494

#Unit 3. Fundamental Processes

U1 and U4 release Formation of the catalytic center in which U6 base pairs with the 5' splice site; U6 also base pairs with U2; U2 remains base paired with the branch site; U5 interacts with both exons through its loop is the characterstic of one of the stage during splicing, which is 

TLS Online TPP Program

#Question id: 24495

#Unit 3. Fundamental Processes

Which of the following step of splicing involves ATP