TLS Online TPP Program

#Question id: 18832


The following PCR technique allows measuring the DNA amplification at each cycle of PCR contrary to end point detection in traditional PCR

#Unit 13. Methods in Biology
  1. q PCR or Quantitative PCR
  2. Reverse transcriptase or RT PCR
  3. Asymmetric PCR
  4. touchdown PCR
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TLS Online TPP Program

#Question id: 10149

#Unit 6. System Physiology – Plant

Formation of supramolecular complexes with regulatory proteins how effects on the catalytic activity of chloroplast enzymes in the dark as well as in the light?

a.) glyceraldehyde-3-phosphate dehydrogenase binds noncovalently with phosphoribulokinase and CP12 form a ternary complex, wherein glyceraldehyde-3-phosphate dehydrogenase and phosphoribulokinase are catalytically inactive in the dark condition

b.) The A2B2 glyceraldehyde-3-phosphate dehydrogenase can form the A8B8 oligomer, Under dark conditions

c.) Reduced thioredoxin cleaves the disulfide bonds of CP12 and phosphoribulokinase, due to the reduction separates the components of the ternary complex releasing the active phosphoribulokinase and the active A4B4-glyceraldehyde 3-phosphate dehydrogenase under the light condition

d.) Reduced thioredoxin (Trx) cleaves the disulfide bond in subunit B of A8B8-glyceraldehyde-3-phosphate dehydrogenase, the reduction converts the active oligomer into the inactive A2B2-glyceraldehyde3-phosphate dehydrogenase under the light condition

Which of the following combination about catalytic activity in dark and the light is true?

TLS Online TPP Program

#Question id: 10150

#Unit 6. System Physiology – Plant

Light controls the assembly of chloroplast enzymes into supramolecular complexes one of the enzyme such as Glyceraldehyde-3-P dehydrogenase, this enzyme acivity is dependent on

TLS Online TPP Program

#Question id: 10151

#Unit 6. System Physiology – Plant

Light regulates the stability of the ternary complex through the ferredoxin–thioredoxin system Regulation of chloroplast phosphoribulokinase and glyceraldehyde-3-phosphate dehydrogenase. Chloroplasts contain two isoforms of glyceraldehyde3-phosphate dehydrogenases, named A4 and A2B2. Which of the following statement about isoform of glyceraldehyde3-phosphate dehydrogenases is False?

TLS Online TPP Program

#Question id: 10152

#Unit 6. System Physiology – Plant

Rubisco catalyzes both the carboxylation and the oxygenation of ribulose 1,5-bisphosphate, the oxygenase activity of rubisco causes partial loss of the carbon fixed by the Calvin–Benson cycle, to avoid loss a reaction takes place such as photorespiration, The following statements about photorespiration.

a.) To avoid drain of carbon out of the Calvin–Benson cycle is metabolized through the C2 oxidative photosynthetic carbon cycle

b.) In photorespiration involves chloroplasts, leaf peroxisomes, and mitochondria

c.) During oxygenation process Rubisco fixes oxygen at high temperature

d.) One of the rection of the C2 cycle is Glycine converted into the serine is takes place in peroxisome

Which of following from above combination is CORRECT?

TLS Online TPP Program

#Question id: 10153

#Unit 6. System Physiology – Plant

Which of the following statements is False?

TLS Online TPP Program

#Question id: 10154

#Unit 6. System Physiology – Plant

The C2 oxidative photosynthetic carbon cycle on chloroplast metabolism, the functioning of the C2 oxidative photosynthetic cycle in three compartments: chloroplasts, peroxisomes and mitochondria such as;

a.) In the carbon cycle; the transfer two molecules of glycolate and recovers one molecule of glycerate and releases one molecule of CO2

b.) In the nitrogen cycle, one molecule of glutamate and recover one molecule of NH4 +

c.) In the oxygen cycle; contributes to the removal of H2O2 formed in the oxidation of glycolate by O2

i) Takes place in Peroxisome

ii) In Mitochondria

iii) In the Chloroplasts

Which of the following combination from the above statements is TRUE?