Practice Questions

TLS Online TPP Program

#Question id: 18851

Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?

#Unit 13. Methods in Biology

Add a low concentration of imidazole to the binding buffer
Gradient elution with increasing imidazole concentration on a chromatography machine
Include protease inhibitors during purification
All of the above

More Questions

#Question id: 7756

#Unit 5. Developmental Biology

#Question id: 29572

#Unit 6. System Physiology – Plant

Which of the following resistant gene provide resistant against C. carbonum infection and also involve in detoxification of HC toxin

#Question id: 26875

#Unit 10. Ecological Principles

Facilitation may be important in

#Question id: 4087

#Unit 3. Fundamental Processes

Polyadenylation can promote translation

a. of the fragile-X gene.

b. of stored oocyte mRNAs.

c. by allowing for greater interaction at the 5´ end.

d. by allowing for greater interaction with translation initiation factors.

#Question id: 12832

#General Aptitude