A good vector must have the following properties.

a) It should be able to replicate autonomously.

b) A vector should be ideally less than 10 kb in size

c) The vector should be easy to isolate and purify.

d)  vector should have suitable marker genes that allow easy detection and/or selection of the transformed host cells.

e) it should a multiple cloning site

#Question id: 596

#Unit 1. Molecules and their Interaction Relevant to Biology

The reason to rewrite the Michaelis-Menten equation (such as the Lineweaver-Burk plot) is to

#Question id: 597

#Unit 1. Molecules and their Interaction Relevant to Biology

The Km values for enzyme reactions such as A + B → C + D

#Question id: 598

#Unit 1. Molecules and their Interaction Relevant to Biology

Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?

#Question id: 599

#Unit 1. Molecules and their Interaction Relevant to Biology

In a certain enzyme-catalyzed reaction the following steps occur:

1. A phosphate group on substrate A is transferred to a side chain of an active site residue of the enzyme.

2. The dephosphorylated form of substrate A dissociates from the enzyme.

3. Substrate B enters the active site and is phosphorylated with simultaneous regeneration of the enzyme in its original form.

What kind of kinetic mechanism is described?

#Question id: 600

#Unit 1. Molecules and their Interaction Relevant to Biology

Two substances are used to produce a certain biological product in an enzyme-catalyzed reaction. It is found that both substrates must bind to the enzyme, first one, then the other before the product is produced. This is an example of a/an ________.

#Question id: 601

#Unit 1. Molecules and their Interaction Relevant to Biology

In a ping-pong reaction which does not occur?