The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

#Question id: 18913

#Unit 13. Methods in Biology

#Question id: 29102

#Unit 8. Inheritance Biology

In mice Igf2 gene which encodes a protein called insulin like growth factor II (Igf2). Offspring inherit one Igf2 allele from their mother and one from their father The paternal copy of Igf2 is actively expressed in the fetus, but the maternal copy is not expressed. which of the following is correct about this gene?

a) this is an example of genomic imprinting and showing maternal imprinted

b) this is an example of genomic imprinting and showing paternal imprinted

c) mechanism of imprinting is due to the addition of methyl (CH3) groups to DNA nucleotides

d) mechanism of imprinting is due to changing the nucleotide sequences

#Question id: 5400

#General Aptitude

a, b, c, d, and e are five consecutive odd numbers then what is the average ?

#Question id: 12672

#Unit 6. System Physiology – Plant

#Question id: 5522

#Unit 5. Developmental Biology

In reptiles, birds, and mammals, mesoderm is made by invagination and involution into the