The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

#Question id: 3808

#Unit 3. Fundamental Processes

Fibroblast cells from several different xeroderma pigmentosa patients were cultured, then fused in intergenic complementation studies. Which cell fusions would not result in a hybrid capable of repairing damaged DNA?

#Question id: 3809

#Unit 3. Fundamental Processes

MMR is a highly conserved biological pathway. Several human MMR proteins have been identified based on their homology to E. coli MMR proteins. Match the following

#Question id: 3810

#Unit 3. Fundamental Processes

In the diagram below, the dotted line marks the point of initiation of bidirectional replication

A. On the right side of the dotted line, leading strand synthesis occurs using the lower strand as the template.

B. On the right side of the dotted line, leading strand synthesis occurs using the upper strand as the template.

C. A ligase deficient (lig- ) mutant would affect replication of t11e upper strand on the left side of the dotted line.

D. A ligase deficient (lig- ) mutant would affect replication of the lower strand on the left side

of the dotted line_

Which one of the following options represents the combinations of the CORRECT statements?

#Question id: 3811

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a characteristic of this replication fork?

#Question id: 3812

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a characteristic of this replication fork?

#Question id: 3813

#Unit 3. Fundamental Processes

The mechanism of DNA replication is studied in an E. coli replication fork. Which is a property of Okazaki pieces in an E. coli replication fork?