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TLS Online TPP Program

#Question id: 18963

The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

#Unit 13. Methods in Biology
  1. MCS
  2. using large size DNA
  3. Co-integrate pTi Vectors
  4. using small size DNA
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TLS Online TPP Program

#Question id: 73

#Unit 1. Molecules and their Interaction Relevant to Biology

Match the Variation of Reaction Spontaneity (Sign of ΔG) given in Column I with the Signs of ΔH and ΔS given in column II.

COLUMN I

COLUMN II

 

 

 

  ΔH

ΔS

ΔG = ΔH – TΔS

1.

−

+

a. The reaction is both enthalpically and entropically opposed. It is nonspontaneous (endergonic) at all temperatures.

2.

 −

− 

b. The reaction is enthalpically opposed (endothermic) but entropically favored. It is spontaneous only at temperatures above T = ΔH/ΔS.

3.

+

+

c. The reaction is enthalpically favored but entropically opposed. It is spontaneous only at temperatures below T = ΔH/ΔS.

4.

+

−

d. The reaction is both enthalpically favored (exothermic) and entropically favored. It is spontaneous (exergonic) at all temperatures.

TLS Online TPP Program

#Question id: 74

#Unit 1. Molecules and their Interaction Relevant to Biology

The enthalpy and entropy of the initial and final states of a reacting system are shown in the table. Calculate the change in free energy for the reaction when the temperature is 4°C.
TLS Online TPP Program

#Question id: 75

#Unit 1. Molecules and their Interaction Relevant to Biology

The experimental approach taken in current biological investigations presumes that ________.
TLS Online TPP Program

#Question id: 76

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following illustrations is not a structural isomer of an organic compound with the molecular formula C6H14? For clarity, only the carbon skeletons are shown; hydrogen atoms that would be attached to the carbons have been omitted.
TLS Online TPP Program

#Question id: 77

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the pairs of molecular structures shown depict enantiomers (enantiomeric forms) of the same molecule?
TLS Online TPP Program

#Question id: 78

#Unit 1. Molecules and their Interaction Relevant to Biology

Which is true about naturally occurring monosaccharides?