TLS Online TPP Program

#Question id: 18980


The most widely used strong regulatable promoters are those

#Unit 13. Methods in Biology
  1. E.coli lac 
  2. trp (tryptophan)
  3. pL, promoter from bacteriophage
  4. All are regulatable promoters 
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TLS Online TPP Program

#Question id: 1725

#Unit 4. Cell Communication and Cell Signaling

What is the single cell type most characteristically associated with the mucosa-associated lymphoid tissue (MALT)?

TLS Online TPP Program

#Question id: 10308

#Unit 6. System Physiology – Plant

Oxidative decarboxylation of pyruvate to acetyl-CoA by the PDH complex, how the pyruvate dehydrogenase complex carries out the five consecutive reactions in the decarboxylation and dehydrogenation of pyruvate?

 A) Pyruvate reacts with the bound thiamine pyrophosphate (TPP) of pyruvate dehydrogenase, undergoing decarboxylation to the hydroxyethyl derivative

 B) A transesterification in which the —SH group of CoA replaces the —SH group of E2 to yield acetyl-CoA and the fully reduced (dithiol) form of the lipoyl group

 C) The reduced FADH2 of E3 transfers a hydride ion to NAD1 , forming NADH, the enzyme complex is now ready for another catalytic cycle

 D) The transfer of two electrons and the acetyl group from TPP to the oxidized form of the lipoyllysyl group of the core enzyme, dihydrolipoyl transacetylase, to form the acetyl thioester of the reduced lipoyl group

 E) Dihydrolipoyl dehydrogenase promotes transfer of two hydrogen atoms from the reduced lipoyl groups of E2 to the FAD prosthetic group of E3 , restoring the oxidized form of the lipoyllysyl group of E2

 Find out the correct sequence of action of this complex enzyme?

TLS Online TPP Program

#Question id: 19294

#Unit 13. Methods in Biology

The various steps for construction of libraries are ___________
i) Fragmentation of DNA
ii) Isolation of genomic DNA
iii) Amplification
iv) Ligation and introduction to the host
v) Vector preparation
The correct order of construction of libraries is (In the order of starting to ending).

TLS Online TPP Program

#Question id: 527

#Unit 1. Molecules and their Interaction Relevant to Biology

What chemical species activates the GLUT4 protein to transport glucose into cells?

TLS Online TPP Program

#Question id: 12387

#Unit 7. System Physiology – Animal

An elderly patient complains of muscle weakness and lethargy. A urine specimen reveals a Na+ concentration of 600 mmol/L and an osmolarity of 1200 mOsm/L. Additional laboratory tests provide the following information: plasma Na+ concentration = 167 mmol/L, plasma renin activity = 4 ng angiotensin I/ml/hr (normal = 1), plasma antidiuretic hormone (ADH) = 60 pg/ml (normal = 3 pg/ml), and plasma aldosterone = 15 ng/dL (normal = 6 ng/dL). Which of the following is the most likely reason for this patient’s hypernatremia?