TLS Online TPP Program

#Question id: 19865


Match the following activity of variants in Column I with their variants of PCR in Column II given below;

                     COLUMN I

                        COLUMN II

 

A) Enhance efficiency

 

i) Multiplex PCR

 

B) Sequence specific amplification

 

ii) Hot-start PCR

 

C) Mutational analysis

 

iii) Megaprimer PCR

 

 

iv) qPCR with SYBR

 

 

v) AS-PCR

 

 

vi) RLM-RACE

#Unit 13. Methods in Biology
  1. A-ii, iv ; B-i, vi ; C-iii, v 
  2. A-iii, v ; B-ii, iv ; C-i, vi
  3. A-i, vi ; B-iii, v ; C-ii, iv
  4. A-ii, iv ; B-i, vi ; C-iii, v
More Questions
TLS Online TPP Program

#Question id: 2233

#Unit 2. Cellular Organization

Membrane proteins may interact with biomembranes through

a. covalently attached fatty acid molecules.

b. covalently attached lipid molecules.

c. noncovalent protein-protein interactions.

d. hydrophobic α-helical domains.

e. noncovalent protein-lipid interactions.

TLS Online TPP Program

#Question id: 2234

#Unit 2. Cellular Organization

Which of the following statement(s) is (are) true of transmembrane proteins?

a. All contains a hydrophobic α helix.

b. Some serve as membrane attachment sites for peripheral membrane proteins.

c. Some contain more than one membrane-spanning domain.

d. All are asymmetrically oriented in the lipid bilayer.

TLS Online TPP Program

#Question id: 2235

#Unit 2. Cellular Organization

The generation and maintenance of a membrane electric potential requires which of the following:

a. active pumping of ions       

b. ion-specific membrane channel proteins

c. ATP                                    

d. a generally permeable membrane

TLS Online TPP Program

#Question id: 2236

#Unit 2. Cellular Organization

The partition coefficient K, the equilibrium constant for partitioning between oil and water, for butyric acid is about 10–2, and for 1,4-butanediol it is about 10–4.  You add liposomes containing only water to a solution with an initial concentration of 1 mM butyric acid and 100 mM 1,4-butanediol outside the liposomes.  What is the relative rate of diffusion of the two substances into the liposome interior?

TLS Online TPP Program

#Question id: 2237

#Unit 2. Cellular Organization

Glucose enters erythrocytes via a GLUT-1 uniporter. As the levels of glucose in the bloodstream decrease between meals, what happens to the glucose in the cells?

TLS Online TPP Program

#Question id: 2238

#Unit 2. Cellular Organization

Consider the transport of glucose into an erythrocyte by facilitated diffusion.  When the glucose concentrations are 5 mM on the outside and 0.1 mM on the inside, the free-energy change for glucose uptake into the cell is:  (These values may be of use to you:  R = 8.315 J/mol·K; T = 298 K; 9 (Faraday constant) = 96,480 J/V; N = 6.022×10²³ /mol.)