TLS Online TPP Program
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TLS Online TPP Program
#Question id: 31031
#Unit 4. Cell Communication and Cell Signaling
Which of the following sequence is correct regarding GPCR signalling pathway?
TLS Online TPP Program
#Question id: 32300
#Unit 1. Molecules and their Interaction Relevant to Biology
Identify the structure shown
TLS Online TPP Program
#Question id: 23031
#Unit 12. Applied Biology
Which of the following portion of some xenobiotic can be traced back to a biological origin to see if these substances were produced oil, natural gas or coal?
TLS Online TPP Program
#Question id: 13090
#Unit 13. Methods in Biology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 3’s plasmid is;
TLS Online TPP Program
#Question id: 32376
#Unit 2. Cellular Organization
Phalloidin isolated from Amanita phalloides, binds to: