Why was it unexpected that transporter proteins are required for transmembrane fatty acid transport?

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#Question id: 18955

#Unit 14. Methods in Biology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

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#Question id: 18956

#Unit 14. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:

 

where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 ⁶ bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 ³ ,with a probability of P will be

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#Question id: 18957

#Unit 14. Methods in Biology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

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#Question id: 18958

#Unit 14. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are

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#Question id: 18959

#Unit 14. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series	I - 300kb - 1,400 kb
B - Cosmids	II - 45 kb
C - BACs and YACs (high capacity vectors)	III - 20kb

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#Question id: 18960

#Unit 14. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library