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TLS Online TPP Program
#Question id: 21671
#Unit 12. Biochemical Engineering
A stirred tank bioreactor of 𝟐.𝟕𝒎𝟑 is agitated using a Rushton turbine with diameter 0.5 m and stirrer speed of 𝟏 𝒔−𝟏. If the fermentation broth has viscosity and density of 𝟏𝟎−𝟐 𝒑𝒂.𝒔 and 𝟏𝟎𝟎𝟎𝒌𝒈.𝒎−𝟑 respectively, the mixing time (in seconds) for the bioreactor will be
TLS Online TPP Program
#Question id: 21678
#Unit 12. Biochemical Engineering
A fermentation medium is being cooled from 700C to 320C in a double pipe heat exchanger. Fluid flowing counter currently with this stream in heated from 200C to 460C. The log mean temperature difference (in0C) for the two streams is
TLS Online TPP Program
#Question id: 21680
#Unit 12. Biochemical Engineering
Consider a bacterium that grows with doubling time of 20 min in the exponential phase of its growth cycle and acquire 10 random mutrations in its genome in every generation. How many mutations will it acquire after 48 hr of growth in exponential phase?
TLS Online TPP Program
#Question id: 21690
#Unit 12. Biochemical Engineering
A bacterial population, growing in batch culture, increases from 1000 cells to 10,00,000 cells in 5 hours. What is the generation time of the bacteria?
TLS Online TPP Program
#Question id: 21741
#Unit 12. Biochemical Engineering
In large scale fermentation processes, corn steep liquor is mainly used as a
TLS Online TPP Program
#Question id: 21742
#Unit 12. Biochemical Engineering
Aqueous two phase partitioining (ATPS) is used for the recovery of an enzyme from the cell free culture fitrate. On addition of PEG-2000 and dextran, the mixture separates into two phases with a partition coefficient for the enzyme as 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases in 5.0, will be
