Reporter genes are used to

a. express enzymes that are not easily assayed in cell extracts.

b. express enzymes that are easily assayed in cell extracts.

c. characterize DNA control elements.                     

d. characterize reporter plasmids.

TLS Online TPP Program

#Question id: 12906

#Unit 13. Methods in Biology

An alternative method labeling strategy used in karyotyping and gene localisation is _______

TLS Online TPP Program

#Question id: 12907

#Unit 13. Methods in Biology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

TLS Online TPP Program

#Question id: 12908

#Unit 13. Methods in Biology

Which statement is incorrect about CRISPR?

TLS Online TPP Program

#Question id: 12909

#Unit 13. Methods in Biology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

TLS Online TPP Program

#Question id: 12910

#Unit 13. Methods in Biology

Match the following:

A. RNA analysis               i. Sexing or cell mutation sites               a. Expressed Sequence tag

B. Forensic science           ii. mRNA analysis                                     b.  Analysis of DNA from blood

C. Gene discovery              iii. DNA profiling                                       c. Active latent viral infections

D. Single-cell analysis     iv.  RT–PCR                                             d. Sex determination of unborn

TLS Online TPP Program

#Question id: 12911

#Unit 13. Methods in Biology

Match the following:

A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis

B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR

C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA

D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond