TLS Online TPP Program

#Question id: 2904


When the temperature of a solution of DNA is raised to near the boiling point of water, the optical density, (called absorbance) at 260 nm markedly increases, a phenomenon known as hyperchromicity. Which of the following properties gets changed due to subsequent denaturation of the DNA molecule?

#Unit 2. Cellular Organization
  1. The solutions of native DNA exhibit high viscosity because of the relatively rigid double helical, long and rod like character of DNA molecule. Denaturation causes a marked decrease in viscosity.

  2. Denaturation of DNA double helix can also be brought about by certain chemical agents such as urea and formamide. These chemical reagents enhance the aqueous solubility of the purine and pyrimidine groups.

  3. Double-stranded DNA shows a strong positive rotation which highly decreases with denaturation. This change is analogous to the change in rotation observed when the proteins are denatured.

  4. All of the above