#Question id: 5292
#Unit 8. Inheritance Biology
A transformation experiment is performed with a donor strain that is resistant to four drugs: W, X, Y, and Z. The recipient is sensitive to all four drugs. The treated recipient population is divided up and plated on media containing various combinations of the drugs. The table below shows the results:
|
Drug(s) added |
Number of Colonies |
|
None |
20,000 |
|
WX |
76 |
|
WY |
1324 |
|
WZ |
1976 |
|
XY |
85 |
|
XZ |
69 |
|
YZ |
1564 |
|
WXY |
50 |
|
WXZ |
62 |
|
WYZ |
1354 |
|
XYZ |
48 |
|
WXYZ |
53 |
Following observation are given
A) X gene is obviously quite distant from the other three genes
B) Y gene is obviously quite distant from the other three genes
C) The Order is three closely link gene W Z Y
D) The Order is three closely link gene W X Z
Which of the following combination is correct?
#Question id: 5293
#Unit 8. Inheritance Biology
The two strains a and b are tested for being auxotrophic for the following nutritional mutations: pyridoxine (pdx), biotin (bio), methionine (met), adenine (ade), and para-aminobenzoic acid (pab). Growth is indicated by ( + ) and lack of growth by ( - ). From these tests, what is genotype of strain a and b respectively (+ indicate prototroph while other auxotroph)?
#Question id: 5294
#Unit 8. Inheritance Biology
Interrupted mating experiments were performed using three different Hfr strains (1-3). The three strains have different combinations of selectable markers. The time of entry for markers for each strain is shown is shown in the table given.
|
Hfr 1 |
Phe (5) |
Trp (35) |
Met (50) |
Ala (52) |
|
Hfr 2 |
Trp (2) |
Val (12) |
His (57) |
Pro (87) |
|
Hfr 3 |
Ala (10) |
Met (12) |
Val (37) |
Phe (57) |
Using the above data, predict the correct sequence of markers on the E. coli chromosome
#Question id: 5295
#Unit 8. Inheritance Biology
A cross is made between an Hfr that is met+ thi+ pur+ and an F-that is met- thi- pur-. Interrupted mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements. These recombinants are tested for the presence of the thi+ and pur+ alleles. The following numbers of individuals are found for each genotype:
|
A) Gene Order |
I |
Thi Pur Met |
|
II |
Pur Met Thi |
|
|
III |
Pur Thi Met |
|
|
B) Distance between Pur and Thi |
IV |
15.4 map unit |
|
V |
1.8 map unit |
What is the following correct combination?
#Question id: 5296
#Unit 8. Inheritance Biology
A transformation experiment is performed with a donor strain that is resistant to four drugs: A, B, C, and D. The recipient is sensitive to all four drugs. The treated recipient cell population is divided up and plated on media containing various combinations of the drugs. The table below shows the results. What is the probable order of the three tightly linked genes?
#Question id: 5297
#Unit 8. Inheritance Biology
In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+thr+ and used to transduce a recipient of genotype cys_ leu_ thr_. Initially, the treated recipient population is plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained. These colonies are then replica-plated onto three different media: What is order of gene?