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TLS Online TPP Program

#Question id: 3415

Imagine a plant of extremely variable height that is pollinated by three different pollinators, one that was attracted to short plants, another that preferred plants of medium height and a third that visited only the tallest plants. If the pollinator that preferred plants of medium height disappeared from an area. What is concluding of this condition?

#Unit 11. Evolution and Behavior

  1. Selection pressures act against plants in the middle of the trait distribution is a result of disruptive natural selection

  2. Selection pressures act against plants in the short and medium of the trait distribution is a result of directional natural selection

  3. Selection pressures act against plants in the short and tall of the trait distribution is a result of stabilized natural selection

  4. Selection pressures random act against plants in the any of the trait distribution is a result of cyclic natural selection

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TLS Online TPP Program

#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program

#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly
A- λ EMBL series                                                                                   I - 300kb - 1,400 kb
B - Cosmids                                                                                                 II - 45 kb
C – BACs and YACs (high capacity vectors)                                             III - 20kb
TLS Online TPP Program

#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library
TLS Online TPP Program

#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 
TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 
TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.
1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.
2. Their large size makes the handling procedures during cloning tedious and cumbersome.
3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned
In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop