Micro-evolution is the term used for changes in allele frequencies that occur over time.

A) It gives rise to changes in the gene pool, which results in few changes in the same species

B) It occur above species level

C) New addition, deletion in the genetic structure, resulting in the formation of new species

D) It changes occurs over short time scales

Which of the following combinations is correct?

TLS Online TPP Program

#Question id: 13074

#Unit 13. Methods in Biology

Type of probe used in RFLP;

TLS Online TPP Program

#Question id: 13075

#Unit 13. Methods in Biology

Radioisotopes must be used in RFLP and RAPD markers

TLS Online TPP Program

#Question id: 13076

#Unit 13. Methods in Biology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 13077

#Unit 13. Methods in Biology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#Unit 13. Methods in Biology

RFLPs have several unique advantages except this one;

TLS Online TPP Program

#Question id: 13079

#Unit 13. Methods in Biology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,