Optimal foraging involves all of the following except

A) maximizing energy gained by the forager.

B) minimizing energy expended by the forager.

C) securing essential nutrients for the forager.

D) minimizing the risk of predation on the forager.

E) maximizing the population size of the forager

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#Question id: 18957

#Unit 13. Methods in Biology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

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#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are

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#Question id: 18959

#Unit 13. Methods in Biology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly

A- λ EMBL series                                                                                   I - 300kb - 1,400 kb

B - Cosmids                                                                                                 II - 45 kb

C – BACs and YACs (high capacity vectors)                                             III - 20kb

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#Question id: 18960

#Unit 13. Methods in Biology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

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#Question id: 18961

#Unit 13. Methods in Biology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called 

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#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting