Start with a collection of mutants obtained using selections or screens and then characterize their wild functions at the molecular level called ----A------, alternatively, start with a set of specific DNA sequences, mutate them, and observe the phenotypes called --B-----.

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#Question id: 18798

#Unit 13. Methods in Biology

which of the is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS.

TLS Online TPP Program

#Question id: 18799

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle. After passing through flow cytometer a graph is observed

  

In this graph A, B, C, and D represent a cell stage which are

TLS Online TPP Program

#Question id: 18800

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle. After passing through flow cytometer a graph is observed

In this double diploid cell are found at 

TLS Online TPP Program

#Question id: 18801

#Unit 13. Methods in Biology

Instead of Propidium iodide-labeled cell an alternate compound can be used to which is

TLS Online TPP Program

#Question id: 18802

#Unit 13. Methods in Biology

Instead of Propidium iodide-labeled cell an alternate compound can be used which is Carboxyfluorescein succinimidyl ester (CFSE) in CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides. The nature of this compound is

TLS Online TPP Program

#Question id: 18803

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE)is non-toxic to cells hence retained till many cycles in CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides, after labelling the following graph is obtained 

 

In this graph