Which of the following correct regarding test cross?

a) A testcross tests reveals the genotype of the individual

b)  Test cross detect linkage between gene

c) Same genotype and phenotype ratio present in test cross progeny

d) Phenotype ratio of test cross used to determine mode of inheritance either complete dominance or incomplete dominance

TLS Online TPP Program

#Question id: 18962

#Unit 13. Methods in Biology

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA,

2. Their large size makes the handling procedures during cloning tedious and cumbersome

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned.

These three problems have been resolved by deleting 

TLS Online TPP Program

#Question id: 18963

#Unit 13. Methods in Biology

The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

TLS Online TPP Program

#Question id: 18964

#Unit 13. Methods in Biology

Size of agrobacterium can be reduced by binary vector formation; these binary vectors can be formed by phenomenon of

TLS Online TPP Program

#Question id: 18978

#Unit 13. Methods in Biology

For expression vector 

TLS Online TPP Program

#Question id: 18979

#Unit 13. Methods in Biology

For expression vector Strong and Regulatable Promoters are used which have

TLS Online TPP Program

#Question id: 18980

#Unit 13. Methods in Biology

The most widely used strong regulatable promoters are those