Which of the following investigators was (were) responsible for the discovery that in DNA from any species, the amount of adenine equals the amount of thymine, and the amount of guanine equals the amount of cytosine?

TLS Online TPP Program

#Question id: 13091

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

TLS Online TPP Program

#Question id: 18661

#Unit 13. Methods in Biology

Which of the following highly sensitive analytical technique particularly useful for studying microheterogeneity in a protein

TLS Online TPP Program

#Question id: 26910

#Unit 10. Ecological Principles

Carnivores are the animals that has the ability to obtaining amino acid by__

TLS Online TPP Program

#Question id: 13001

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following is incorrect 

a) antiparallel β sheets are frequently found and they are quite more stable because of more no. hydrogen bond presence and linear H bonding pattern.

b) antiparallel β sheets are frequently found and they are quite more stable because of more no. hydrogen bond presence and having distorted H bonding pattern.

c) parallel β sheets are relatively less stable and mostly found in core of protein.

d) parallel β sheets are relatively more stable and mostly found in core of protein.

TLS Online TPP Program

#Question id: 229

#Unit 1. Molecules and their Interaction Relevant to Biology

Rickets in children and osteomalacia in adults is caused by a lack of ________.