In E. coli, which enzyme catalyzes the elongation of a new DNA strand in the 5' → 3' direction?

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#Question id: 13064

#Unit 13. Methods in Biology

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 28923

#Unit 4. Cell Communication and Cell Signaling

Which of the following are seven TM α helices and is a member of the GPCR Superfamily?

a) RYK and ROR

b) Smoothened (Smo)

c) Patched (Ptc)

d) Frizzled (Fz)

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#Question id: 300

#Unit 1. Molecules and their Interaction Relevant to Biology

Hydrogen bonding occurs in molecules when ___________________.

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#Question id: 3806

#Unit 3. Fundamental Processes

How are damaged nucleotides recognized by DNA repair enzymes?

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#Question id: 23664

#Unit 13. Methods in Biology

________ is a technique used to link  noncontiguous recombinants from the same gene or to identify genes whose sequence and gene product are unknown other than by the effects of mutation.