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TLS Online TPP Program

#Question id: 23666

Which is not necessary as part of an expression vector used in E. coli?

#Unit 13. Methods in Biology
  1. marker gene 
  2. ribosome binding site
  3. Taq polymerase gene 
  4. strong promoter sequence
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TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program

#Question id: 5002

#Unit 11. Evolution and Behavior

All animals with eyes or eyespots that have been studied so far share a gene in common. When mutated, the gene Pax-6 causes lack of eyes in fruit flies, tiny eyes in mice, and missing irises (and other eye parts) in humans. The sequence of Pax-6 in humans and mice is identical. There are so few sequence differences with fruit fly Pax-6 that the human/mouse version can cause eye formation in eyeless fruit flies, even though vertebrates and invertebrates last shared a common ancestor more than 500 million years ago.

The appearance of Pax-6 in all animals with eyes can be explained in multiple ways. Based on the information above, which explanation is most likely?
TLS Online TPP Program

#Question id: 23874

#Unit 13. Methods in Biology

Scientists developed a set of guidelines to address the safety of DNA technology. Which of the following is one of the adopted safety measures?
TLS Online TPP Program

#Question id: 4924

#Unit 8. Inheritance Biology

Following statement given for a character

A- Characteristics based on autosomal genes

B- Biparntal inheritance

C-pattern of gene expression depend on parental origin

Which of the following having above statements feature?
TLS Online TPP Program

#Question id: 14686

#Unit 4. Cell Communication and Cell Signaling

This member of the AKAP family, designated mAKAP, anchors both PDE and the regulatory subunit  of PKA to the nuclear membrane, maintaining them in a negative feedback loop that provides close local control of the ATP level and PKA activity.
B- The basal level of PDE activity in the presence of hormone (resting state) keeps cAMP levels below those necessary for PKA activation. 
C- Activation of β-adrenergic receptors causes an increase in cAMP to a level in excess of that which can be degraded by PDE. 
D- The resulting binding of cAMP to the R subunits of PKA releases the active catalytic (C) subunits into the cytosol. Some C subunits enter the nucleus, where they phosphorylate and thus activate certain transcription factors . Other C subunits phosphorylate PDE, stimulating its catalytic activity.
E-  Active PDE hydrolyzes cAMP, thereby driving cAMP levels back to basal levels and causing re-formation of the inactive PKA C-R complex. Subsequent de-phosphorylation of PDE returns the complex to the resting state.