What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I. Transform bacteria with recombinant DNA molecule.

II. Cut the plasmid DNA using restriction enzymes.

III. Extract plasmid DNA from bacterial cells.

IV. Hydrogen-bond the plasmid DNA to non plasmid DNA fragments.

V. Use ligase to seal plasmid DNA to non plasmid DNA.

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#Question id: 4759

#Unit 8. Inheritance Biology

which of the following is not a mechanism for sex determination?

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#Question id: 28979

#Unit 7. System Physiology – Animal

autoimmune destruction of all zones of the adrenal cortex causes___

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#Question id: 18782

#Unit 13. Methods in Biology

The concentration of a small amount antibodies 2 μg antibody/ml can be detected

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#Question id: 2963

#Unit 2. Cellular Organization

Eukaryotic chromosomes are composed of which of the following macromolecules?

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#Question id: 518

#Unit 1. Molecules and their Interaction Relevant to Biology

Transfer of the phosphoryl group from PEP to ADP is an example of