TLS Online TPP Program

#Question id: 23690


A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that

#Unit 13. Methods in Biology
  1. prokaryotes use a different genetic code from that of eukaryotes.
  2. bacteria translate polycistronic messages only.
  3. bacteria cannot remove eukaryotic introns.
  4. bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
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TLS Online TPP Program

#Question id: 4282

#Unit 3. Fundamental Processes

Which of the following protein(s) is (are) found in the peroxisome matrix?

TLS Online TPP Program

#Question id: 4283

#Unit 3. Fundamental Processes

Proteins can enter which organelle(s) in their folded conformation?

TLS Online TPP Program

#Question id: 4284

#Unit 3. Fundamental Processes

Assays for following the trafficking of proteins through the secretory pathway in living cells require

a. sensitive methods to distinguish the very small differences in organelle density caused by passage of a wave of protein transport through the organelle.

b. a way to identify compartments where labeled proteins are located.

c. the use of cell types that are highly dedicated secretory cells.

d. the use of enucleate cells in order to eliminate the nucleus as a site of labeling.

e. a way to label a cohort of secretory proteins

TLS Online TPP Program

#Question id: 4285

#Unit 3. Fundamental Processes

VSV G protein has been a very useful tracker protein for both living cell and in vitro assays because

TLS Online TPP Program

#Question id: 4286

#Unit 3. Fundamental Processes

Transport vesicles

a. can have a clathrin coat.     b. can be coated with a heteromeric COPI and COPII coat.

c. can contain assembly proteins.

d. can shuttle proteins from mitochondria to the endoplasmic reticulum.

e. can fuse with target organelles.

TLS Online TPP Program

#Question id: 4287

#Unit 3. Fundamental Processes

How the mTOR pathway increases the protein synthesis?