TLS Online TPP Program

#Question id: 23690


A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that

#Unit 13. Methods in Biology
  1. prokaryotes use a different genetic code from that of eukaryotes.
  2. bacteria translate polycistronic messages only.
  3. bacteria cannot remove eukaryotic introns.
  4. bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.