TLS Online TPP Program

#Question id: 23708


In order to identify a specific restriction fragment using a probe, what must be done?

#Unit 13. Methods in Biology
  1. The fragments must be separated by electrophoresis.
  2. The fragments must be treated with heat or chemicals to separate the strands of the double helix.
  3. The probe must be hybridized with the fragment.
  4. A, B, and C are correct.
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TLS Online TPP Program

#Question id: 598

#Unit 1. Molecules and their Interaction Relevant to Biology

Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?

TLS Online TPP Program

#Question id: 599

#Unit 1. Molecules and their Interaction Relevant to Biology

In a certain enzyme-catalyzed reaction the following steps occur:

1. A phosphate group on substrate A is transferred to a side chain of an active site residue of the enzyme.

2. The dephosphorylated form of substrate A dissociates from the enzyme.

3. Substrate B enters the active site and is phosphorylated with simultaneous regeneration of the enzyme in its original form.

What kind of kinetic mechanism is described?

TLS Online TPP Program

#Question id: 600

#Unit 1. Molecules and their Interaction Relevant to Biology

Two substances are used to produce a certain biological product in an enzyme-catalyzed reaction. It is found that both substrates must bind to the enzyme, first one, then the other before the product is produced. This is an example of a/an ________.

TLS Online TPP Program

#Question id: 601

#Unit 1. Molecules and their Interaction Relevant to Biology

In a ping-pong reaction which does not occur?

TLS Online TPP Program

#Question id: 602

#Unit 1. Molecules and their Interaction Relevant to Biology

An inhibitor binds to a site other than the active site of the enzyme. Which statement below correlates with this observation?

TLS Online TPP Program

#Question id: 603

#Unit 1. Molecules and their Interaction Relevant to Biology

Nonclassical competitive inhibition involves ________.