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TLS Online TPP Program

#Question id: 23844

Thin-filament regulation is,

#Unit 2. Cellular Organization
  1. Dynein-dependent mechanism which regulate contraction in smooth muscle and non-muscle cells. 
  2. Actin & Myosin-dependent mechanisms which regulate contraction in skeletal muscles.
  3. Kinesin-dependent mechanism which regulate contraction in skeletal muscle. 
  4. Actin-dependent mechanism which regulate contraction in smooth muscle and non-muscle cells.
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TLS Online TPP Program

#Question id: 4219

#Unit 3. Fundamental Processes

Which of the following statements correctly describes Archibald Garrod's hypothesis for how "inborn errors of metabolism" such as alkaptonuria occur?
TLS Online TPP Program

#Question id: 13175

#Unit 10. Ecological Principles

Which of the following biomes is characterized by a wet, mild winter and a warm, dry summer? 
TLS Online TPP Program

#Question id: 18855

#Unit 13. Methods in Biology

to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?
TLS Online TPP Program

#Question id: 2908

#Unit 2. Cellular Organization

The H3.H4 tetramers and H2A.H2B dimers each interact with a particular region of the DNA within the nucleosome. Which of the following statement is CORRECT regarding the characteristic binding of histones with the DNA?

I. Of the 147 bp of DNA included in the structure, the histone-fold regions of the H2A.H2B dimer interact with the central 60 bp.

II. Histone H3.H4 tetramers occupy a key position in the nucleosome by binding the middle and both ends of the DNA.

III. The amino-terminal region of H4 most proximal to the histone-fold region forms a fourth alpha helix that interacts with the final 13 bp at each end of the bound DNA.

IV. The H3.H4 tetramer associate with approximately 30 bp of DNA on either side of the central 60 bp of DNA bound by H2A.H2B dimer.

V. The two H2A.H2B dimers form the bottom part of the histone octamer located across the disc from the DNA ends.
TLS Online TPP Program

#Question id: 13090

#Unit 13. Methods in Biology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 3’s plasmid is;