#Question id: 5281
#Unit 8. Inheritance Biology
In the mating Hfr Strs met+ his+ leu+ trp+ Ala+ X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:
|
Interrupted time |
Composition of media |
Growth |
||||
|
His |
Leu |
Ala |
Trp |
Met |
||
|
2 min |
+ |
- |
+ |
+ |
+ |
Yes |
|
10 min |
+ |
- |
- |
+ |
+ |
Yes |
|
12 min |
+ |
- |
- |
+ |
- |
No |
|
15 min |
+ |
- |
- |
- |
+ |
Yes |
|
20 min |
- |
- |
- |
- |
+ |
Yes |
What is the order of transfer of the genes?
#Question id: 5282
#Unit 8. Inheritance Biology
Conjugation allowed between Hfr with genotype A B C D E and F- with genotype a b c d e. At regular intervals, a sample of cells was removed and agitated
vigorously in a kitchen blender to halt all conjugation and DNA transfer. After interrupted conjugation, ex-conjugate were selected on minimal media at regular interval and find different number of ex-conjugate?
abCde - 10, abCdE - 30 , aBCdE - 40 , ABCdE - 20 , ABCDE - 50
What is gene order of those genes?
#Question id: 5283
#Unit 8. Inheritance Biology
A generalized transducing phage is used to transduce an a- b- c- d- e- recipient strain of E. coli with an a+ b+c+d+ e+ donor. The recipient culture is plated on various media with the results shown in the table below What can you conclude about the linkage and order of the genes?
#Question id: 5284
#Unit 8. Inheritance Biology
DNA from a strain of Bacillus subtilis with genotype a+b+ c+d+ e+ is used to transform a strain with genotype a - b- c- d- e-. Pairs of genes are checked for co-transformation and the following results are obtained:
Pair of co-transform genes as follow
D-E, E-C, C-B, A-D
On the basis of these results, what is the order of the genes on the bacterial chromosome?
#Question id: 5285
#Unit 8. Inheritance Biology
A transformation experiment is carried out using donor DNA that is A+B+C+ and a recipient that is A-B- C-. A+ transformants are selected and tested further. Of these, 64% are B+ and none are C+. Also, B+ are selected, and 8% are also C+. What is the gene order
#Question id: 5286
#Unit 8. Inheritance Biology
An Hfr strain with genotype A B C D (each gene for amino acid synthesis )and that transfers the A gene very late was mated with recipient strain with genotype a b c d. After mating for several hours, the mixture was diluted and plated on minimal media with four different supplements having various number of colony
Media supplement with amino acid
Colony number
A+C 500
C+D 50
B 10
A 250
What is order of gene