Gene therapy

TLS Online TPP Program

#Question id: 4482

#Unit 3. Fundamental Processes

The General Transcription Factors of RNA Polymerase, with their subunits, select correct matching

A.     TBP	1
B.     TFIA	11
C.     TAFs	2
D.     TFIIH	10

TLS Online TPP Program

#Question id: 4483

#Unit 3. Fundamental Processes

TAF42 and TAF62 from Drosophila form a structure similar to that of the

TLS Online TPP Program

#Question id: 4484

#Unit 3. Fundamental Processes

Match General Transcription Factors with their functions;

i. TAFs	A. Appears to regulate the binding of TBP to DNA. It does this using an inhibitory flap that binds to the DNA-binding surface of TBP, another example of molecular mimicry. This flap must be displaced for TBP to bind TATA.
ii. TFIIB	B. Insert into the RNA-exit channel and active center cleft of Pol II in a manner analogous to the sigma region 3/4 linker in the bacterial case.
iii. TFIIH	C.  As an ATP-driven translocator of double-stranded DNA.
iv. TFIIF	D. This two-subunit (in humans) factor associates with Pol II and is recruited to the promoter together with that enzyme.

TLS Online TPP Program

#Question id: 4485

#Unit 3. Fundamental Processes

In multicellular organisms, regulatory sequences can spread thousands of nucleotides from the promoter—both upstream and downstream—and can be made up of tens of regulator binding sites. Often, these binding sites are grouped in units called_?

TLS Online TPP Program

#Question id: 4486

#Unit 3. Fundamental Processes

Activators can recruit factors needed for polymerase to initiate or elongate transcription. In all of these functions, the activator is merely recruiting proteins to the promoter. In bacteria, RNA polymerase is the only protein that needs to be recruited; this is not the case in eukaryotes. Indeed, in eukaryotes, a given activator might work in all three ways:

1.    Recruitment of nucleosome modifiers and remodelers	i.     “open” the promoter
2.    Recruitment of general transcription factors and mediators	ii.      That stimulate Pol II initiation and elongation
3.       Recruitment of protein complexes	iii.     To recruit PTEFb/SEC

TLS Online TPP Program

#Question id: 4487

#Unit 3. Fundamental Processes

Choose correct statements about nucleosome modifiers