TLS Online TPP Program

#Question id: 3818


DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

If the mechanism of DNA replication in this bacterium were semi-conservative, what results would be found when the double-stranded DNA was analyzed in a CsCl density gradient after three generations in 15N-medium?

#Unit 3. Fundamental Processes
  1. The DNA would form three bands: one with hybrid density, one with light density, and one with heavy density.

  2. The DNA would form one band with hybrid density.

  3. The DNA would form two bands: one with heavy density and one with hybrid density.

  4. The DNA would form two bands: one with light density and one with hybrid density.