TLS Online TPP Program

#Question id: 3819


DNA replication is studied in a newly discovered bacterium. It takes 30 min for the bacterium to complete a round of replication at 37oC. A Meselson-Stahl-type experiment was performed. The bacteria were grown for several generations in 14N-medium and then switched to 15N-medium. The DNA molecules were then analyzed.

After one generation in 15N-medium, the DNA is denatured and analyzed in a CsCl gradient. If the single-stranded DNA forms two bands, one with heavy density and one with light density, what conclusion could be drawn about the mechanism of DNA replication in this bacterium?

#Unit 3. Fundamental Processes
  1. The mechanism must be conservative.

  2. The mechanism must be semi-conservative

  3. The mechanism could be either conservative or semi-conservative.

  4. No conclusions about the mechanism can be made by analyzing single stranded DNA.