State whether the following statements is true/false regarding DNA replication.

1. Proteins bind to both origin sites and termination sites on DNA during DNA replication.

2. In the Sanger method for DNA sequencing, the gels are read from bottom to top, since the smallest fragments travel farthest in the sequencing gel.

3. DNA polymerase I which is inhibited by dideoxynucleoside triphosphates is used in the Sanger method for DNA sequencing.

4. Sanger DNA sequencing requires inhibition of DNA polymerization by a specific dideoxynucleoside every time it is copied in the replication process.

5. The Klenow fragment can be used for DNA sequencing if proteins such as SSB protein are added.

#Question id: 2446

#Unit 2. Cellular Organization

The dynactin protein complex serves to

a. link vesicles and chromosomes to cytosolic dynein light chains.

b. link axonemal dynein to B tubules.

c. enhance the processivity of cytosolic dynein.

d. mediate microtubule attachment to centrosomes.

e. attach kinesin to vesicles.

#Question id: 2447

#Unit 2. Cellular Organization

In the axonemes, the arrangement of nine outer doublet microtubules, as well as the protofilament number of each outer doublet, is specified by

#Question id: 2448

#Unit 2. Cellular Organization

Axonemal dynein

a. may contain two or three head domains.

b. attaches to a B tubule through its base domain.

c. consists of heavy, intermediate and light chains.

d. is a (-) end-directed motor.            

e. is an ATPase.

#Question id: 2449

#Unit 2. Cellular Organization

What is the correct order of the stages of mitosis?

a. anaphase               

 b. metaphase              

c. telophase    

d. prophase                 

e. prometaphase

#Question id: 2450

#Unit 2. Cellular Organization

Which of the following contribute(s) to pole separation during anaphase B?

a. cytosolic dynein     

b. a (-) end-directed kinesin               

c. CENP-E                     

d. microtubule depolymerisation                   

e. microtubule polymerization

#Question id: 2451

#Unit 2. Cellular Organization

The average lifetime of microtubules during mitosis is