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TLS Online TPP Program

#Question id: 24225

Choose the incorrect option

#Unit 1. Molecules and their Interaction Relevant to Biology
  1. There are two kind of pyruvate kinase 1. Liver isozyme (L form), 2. Muscle isozyme (M form)
  2. The liver isozyme (L form) is also regulated hormonally by glucagon. Glucagon to PKA mediated phosphorylation inactivate Pyruvate Kinase L.
  3. The muscle isozyme (M form) PK is not affected by this phosphorylation mechanism.
  4. Glucagon increases the cellular level of fructose 2,6 bisphosphate, stimulating glycolysis and inhibiting gluconeogenesis.
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TLS Online TPP Program

#Question id: 12910

#Unit 13. Methods in Biology

Match the following:
A. RNA analysis               i. Sexing or cell mutation sites               a. Expressed Sequence tag
B. Forensic science           ii. mRNA analysis                                     b.  Analysis of DNA from blood
C. Gene discovery              iii. DNA profiling                                       c. Active latent viral infections
D. Single-cell analysis     iv.  RT–PCR                                             d. Sex determination of unborn
TLS Online TPP Program

#Question id: 12911

#Unit 13. Methods in Biology

Match the following:
A. DNA polymerases              i. Alkaline phosphatase        a.RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase        ii. T4 DNA ligase                    b. Thermostable DNA polymerase used in PCR
C. Ligases                                 iii.  AMV-RT                            c. Removes 5′-phosphates from DNA and RNA
D. Phosphatases                     iv. Taq DNA pol                      d. Links 5′-phosphate and 3′-hydroxyl ends via phosphodiester bond
TLS Online TPP Program

#Question id: 12912

#Unit 13. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program

#Question id: 12913

#Unit 13. Methods in Biology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 
TLS Online TPP Program

#Question id: 12914

#Unit 13. Methods in Biology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________
TLS Online TPP Program

#Question id: 12915

#Unit 13. Methods in Biology

Which of the following statement is not correct about PCR.