Which of the following proteins does not “footprint” the lac operon control region?

TLS Online TPP Program

#Question id: 18848

#Unit 13. Methods in Biology

You find that your protein sample loses activity during storage. What can you do about this?

TLS Online TPP Program

#Question id: 18849

#Unit 13. Methods in Biology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?

TLS Online TPP Program

#Question id: 18850

#Unit 13. Methods in Biology

Which of these chromatography types are suitable as a "capture" step in the purification of non-tagged proteins?

TLS Online TPP Program

#Question id: 18851

#Unit 13. Methods in Biology

Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?

TLS Online TPP Program

#Question id: 18852

#Unit 13. Methods in Biology

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

TLS Online TPP Program

#Question id: 18853

#Unit 13. Methods in Biology

Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?