Many eukaryotic genes contain a large number of exons. Correct splicing of such genes requires that neighboring exons be ligated to one another; if they are not, exons will be left out. One early proposal suggested that the splicing machinery bound to a splice site at one end of an intron and scanned through the intron to find the splice site at the other end. Such a scanning mechanism would guarantee that an exon was never skipped. This hypothesis was tested with one minigene with a duplicated 3ʹ splice site. Find diagram of the products you expect from minigene if the splicing machinery binds to a 3ʹ splice site and scans toward a 5ʹ splice site.

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#Question id: 8893

#Unit 9. Diversity of Life Forms

While sampling marine plankton in a lab, a student encounters large numbers of fertilized eggs. The student rears some of the eggs in the laboratory for further study and finds that the blastopore becomes the mouth. The embryo develops into a trochophore larva and eventually has a true coelom. These eggs probably belonged to a(n)

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#Question id: 4560

#Unit 3. Fundamental Processes

TFIIH general is a general transcription factor phosphorylates the CTD multiple times on heptapeptide sequence such as

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#Question id: 13058

#Unit 13. Methods in Biology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 5166

#General Aptitude

Which of the following is the largest ?

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#Question id: 5297

#Unit 8. Inheritance Biology

In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+thr+ and used to transduce a recipient of genotype cys_ leu_ thr_. Initially, the treated recipient population is plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained. These colonies are then replica-plated onto three different media: What is order of gene?