The cAMP receptor protein (CRP) is said to be responsible for positive regulation of the lac operon because __.

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#Question id: 18953

#Unit 13. Methods in Biology

These membranes are made on Teflon base: they have high affinity for proteins, are highly mechanically stable like nylon membranes and can be used for provides high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods.  

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#Question id: 18954

#Unit 13. Methods in Biology

The above diagram showing which of the following technique

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#Question id: 18955

#Unit 13. Methods in Biology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

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#Question id: 18956

#Unit 13. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:

 

where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 ⁶ bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 ³ ,with a probability of P will be

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#Question id: 18957

#Unit 13. Methods in Biology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

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#Question id: 18958

#Unit 13. Methods in Biology

The number of clones to represent this fragment in genomic library with a probability of 95% are