Give correct explanation, why RNA polymerase can initiate a new RNA chain on a DNA template without any primer?

A. Because RNA polymerase starts most transcripts with an A, and that ribonucleotide binds the template nucleotide (T) with only two hydrogen bonds.

B. The enzyme has to make specific interactions with one or all of the DNA template strand, the initiating ribonucleotide, and the second ribonucleotide—holding one (or all) rigidly in the correct orientation to allow chemical attack on the incoming NTP.

C. The structure of the open complex shows that the s region 3/4 linker interacts with the template strand, organizing it in the correct conformation and location to allow initiation.

D. Consistent with this, in experiments using an RNA polymerase containing a s70 derivative lacking this part of s, initiation requires much lower than normal concentrations of one or both of the first two ribonucleotides.

TLS Online TPP Program

#Question id: 19103

#Unit 4. Cell Communication and Cell Signaling

During infection, most bacteria remain in extracellular spaces and use a specialized mechanism, to deliver bacterial effector proteins into the plant cell that is

TLS Online TPP Program

#Question id: 19103

#Unit 6. System Physiology – Plant

During infection, most bacteria remain in extracellular spaces and use a specialized mechanism, to deliver bacterial effector proteins into the plant cell that is

TLS Online TPP Program

#Question id: 19104

#Unit 4. Cell Communication and Cell Signaling

The structural components of the T3SS are encoded by the clustered gene known as

TLS Online TPP Program

#Question id: 19104

#Unit 6. System Physiology – Plant

The structural components of the T3SS are encoded by the clustered gene known as

TLS Online TPP Program

#Question id: 19105

#Unit 4. Cell Communication and Cell Signaling

The type III secretion system (T3SS), to deliver bacterial effector proteins into the plant cell have been identified to be secretion of enzymes which causes dead of the plant, these enzymes are;

a) Phosphatases

b) Proteases 

c)  Lyases

d) Isomerase

e) Ligase

TLS Online TPP Program

#Question id: 19105

#Unit 6. System Physiology – Plant

The type III secretion system (T3SS), to deliver bacterial effector proteins into the plant cell have been identified to be secretion of enzymes which causes dead of the plant, these enzymes are;

a) Phosphatases

b) Proteases 

c)  Lyases

d) Isomerase

e) Ligase