The large subunit of Pol II has a carboxy-terminal domain (CTD), which is referred to as the “tail”. The CTD contains a series of repeats of the heptapeptide sequence is;

TLS Online TPP Program

#Question id: 18978

#Unit 13. Methods in Biology

For expression vector 

TLS Online TPP Program

#Question id: 18979

#Unit 13. Methods in Biology

For expression vector Strong and Regulatable Promoters are used which have

TLS Online TPP Program

#Question id: 18980

#Unit 13. Methods in Biology

The most widely used strong regulatable promoters are those

TLS Online TPP Program

#Question id: 18981

#Unit 13. Methods in Biology

The pL promoter is controlled by the cl repressor protein of bacteriophage A. In practice, a temperature-sensitive mutant of the cl repressor, cl857, is generally used to regulate pL-directed transcription. Cells carrying the temperature-sensitive cl repressor are first grown at 30°C, and 42°C

TLS Online TPP Program

#Question id: 18982

#Unit 13. Methods in Biology

A number of convenient vector systems that incorporate both transcriptional and translational signals for the expression of cloned eukaryotic genes in E. coli have been developed. One such system is called expression vector is 

TLS Online TPP Program

#Question id: 18983

#Unit 13. Methods in Biology

which of the following is biggest problem for translation efficiency