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TLS Online TPP Program

#Question id: 451

The standard reduction potentials (E'°) for the following half reactions are given.

Fumarate + 2H+ + 2e– -> succinate E'° = +0.031 V

FAD + 2H+ + 2e– -> FADH2 E'° = –0.219 V

If you mixed succinate, fumarate, FAD, and FADH2 together, all at l M concentrations and in the presence of succinate dehydrogenase, which of the following would happen initially?

#Unit 1. Molecules and their Interaction Relevant to Biology

  1. Fumarate and succinate would become oxidized; FAD and FADH2 would become reduced.

  2. Fumarate would become reduced, FADH2 would become oxidized.

  3. No reaction would occur because all reactants and products are already at their standard concentrations.

  4. Succinate would become oxidized, FAD would become reduced.

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TLS Online TPP Program

#Question id: 13071

#Unit 13. Methods in Biology

Modifications of the agglutination reaction involve the use of ________particles, which allow the reaction to be observed more easily in the liquid phase
TLS Online TPP Program

#Question id: 13072

#Unit 13. Methods in Biology

Inheritance pattern of RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13073

#Unit 13. Methods in Biology

Restriction enzymes must be use in RFLP and RAPD markers for genome mapping in plants;
TLS Online TPP Program

#Question id: 13074

#Unit 13. Methods in Biology

Type of probe used in RFLP;
TLS Online TPP Program

#Question id: 13075

#Unit 13. Methods in Biology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Unit 13. Methods in Biology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.